Anti-Mitofilin antibody
| 英文名称 | Mitofilin |
| 中文名称 | 线粒体内膜蛋白抗体 |
| 别 名 | Cell proliferation inducing protein 52; Cell proliferation-inducing gene 4/52 protein; HMP; IMMT; IMMT_HUMAN; Inner membrane protein mitochondrial; Inner mitochrondial membrane; MINOS2; Mitochondrial inner membrane organizing system 2; Mitochondrial inner membrane protein; Mitofilin; Motor protein; p87; p87/89; p89; pig4; PIG52; Proliferation-inducing gene 4; MICOS complex subunit MIC60. |
DATASHEET
Host:Rabbit
Target Protein:Mitofilin
IR:Immunogen Range:241-340/758
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10989
Swiss Prot:Q16891
Source:KLH conjugated synthetic peptide derived from human Mitofilin:241-340/758
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Mitochondria are the center of cellular energy production and essential metabolic reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology. Mitofilin was originally described as heart muscle protein (HMP) because of its high expression in the heart.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-Mitofilin (bs-1824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (bs-1824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (bs-1824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (bs-1824R) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr. This image was kindly provided by an end-user.
Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (bs-1824R) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr. This image was kindly provided by an end-user.
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