Anti-TEM1 antibody (bs-2101R)

Anti-TEM1 antibody

DATASHEET

Host:Rabbit

Target Protein:TEM1

IR:Immunogen Range:251-350/757

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57124

Swiss Prot:Q9HCU0

Source:KLH conjugated synthetic peptide derived from human TEM 1:251-350/757 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The transmembrane glycoprotein Tumor endothelial marker 1 (TEM1) is highly expressed in tumor endothelial cells however it is barely detectable on normal endothelial cells. It is believed to play a role in tumor angiogenesis. It is expressed in stromal fibroblasts, metastatic hepatic lesions and during angiogenesis of corpus luteum formation and wound healing. TEM1 is being investigated as a potential target for cancer treatment.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Thymus(mouse) Lysate at 40 ug Primary: Anti-TEM1 (bs-2101R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kD

Sample: spleen(mouse) Lysate at 30 ug
Primary: Anti-TEM1 (bs-2101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 130 kD

Sample:
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti-TEM1 (bs-2101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 130 kD

Sample:
SW480(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti-TEM1 (bs-2101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 130 kD

Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEM1) Polyclonal Antibody, Unconjugated (bs-2101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.