Anti-CD44v4 antibody
| 英文名称 | CD44v4 |
| 中文名称 | CD44V4抗体 |
| 别 名 | CD44 antigen; CD44 antigen isoform 4 precursor; CD44 molecule; Heparan sulfate proteoglycan; PGP1; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MGC10468; MIC4; Pgp1; CD44 antigen isoform 4 precursor; hematopoietic cell E- and L-selectin ligand; chondroitin sulfate proteoglycan 8; cell surface glycoprotein CD44; GP90 lymphocyte homing/adhesion receptor; heparan sulfate proteoglycan; hyaluronate receptor; Hermes antigen; CD44 antigen; homing function and Indian blood group system; epican; phagocytic glycoprotein 1; extracellular matrix receptor III. |
DATASHEET
Host:Rabbit
Target Protein:CD44v4
IR:Immunogen Range:141-240/361
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:960
Swiss Prot:P16070
Source:KLH conjugated synthetic peptide derived from human CD44v4:141-240/361
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:WB()
ELISA(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /Test)
ICC(1:100)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Dog
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Bone (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD44v4 (bs-2783R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 50 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-CD44v4 (bs-2783R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (CD44v4) polyclonal Antibody, Unconjugated (bs-2783R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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