Anti-phospho-P53 (Ser46) antibody (bs-3707R)

Anti-phospho-P53 (Ser46) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-P53 (Ser46)

IR:Immunogen Range:ML(p-S)PD

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7157

Swiss Prot:P04637

Source:KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Ser46:ML(p-S)PD 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(0.2μg /test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-P53(Ser46) (bs-3707R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-P53 (Ser46) (bs-3707R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 55 kD

Sample:
A549(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-P53(Ser46) (bs-3707R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD

Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-P53(Ser46) Polyclonal Antibody, Unconjugated(bs-3707R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining