Anti-phospho-IGF1R (Tyr980) antibody
| 英文名称 | phospho-IGF1R (Tyr980) |
| 中文名称 | 磷酸化胰岛素样生长因子1受体抗体 |
| 别 名 | IGF1 Receptor (phospho Y980); p-IGF1 Receptor (phospho Y980); IGF1R (phospho-Tyr980); IGF1R (phospho-Y980); p-IGF1R (Tyr980); p-IGF1R (Y980); IGF1R (phospho Tyr980); CD221; CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF 1R; IGF I receptor; IGF-I receptor; IGF1R; IGF1R; IGF1R_HUMAN; IGFIR; IGFIRC; IGFIRC; IGFR; Insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor precursor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; JTK13; MGC142170; MGC142172; MGC18216; IGF-IR. |
DATASHEET
Host:Rabbit
Target Protein:phospho-IGF1R (Tyr980)
IR:Immunogen Range:PE(p-Y)FS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3480
Swiss Prot:P08069
Source:KLH conjugated Synthesised phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr980:PE(p-Y)FS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: human kidney carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-IGF1R(Tyr980) Polyclonal Antibody, Unconjugated(bs-5447R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RELM beta) Polyclonal Antibody, Unconjugated (bs-5774R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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