Anti-PKM2 antibody
| 英文名称 | PKM2 |
| 中文名称 | 丙酮酸激酶-M2抗体 |
| 别 名 | PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PKM2; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; Tumor M2-PK; p58; OIP-3; KPYM_HUMAN. |
DATASHEET
Host:Mouse
Target Protein:PKM2
IR:Immunogen Range:51-150/531
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5315
Swiss Prot:P14618
Source:KLH conjugated synthetic peptide derived from human PKM2:51-150/531
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: U87MG (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-PKM2 (bs-0102M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-PKM2 (bs-0102M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample:
Hela Cell (Human) Lysate at 40 ug
NIH/3T3 Cell (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Jurkat Cell (Human) Lysate at 40 ug
Primary: Anti-PKM2 (bs-0102M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Tissue/cell: Human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated(bs-0102M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
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