Anti-Latency-associated peptide antibody (bs-4908R)

Anti-Latency-associated peptide antibody

DATASHEET

Host:Rabbit

Target Protein:Latency-associated peptide

IR:Immunogen Range:101-200/390

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7040

Swiss Prot:P01137

Source:KLH conjugated synthetic peptide derived from human Latency-associated peptide:101-200/390 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Latency-associated peptide (bs-4908R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30/44 kD
Observed band size: 30 kD

Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: K562 (Human) Cell Lysate at 30 ug
Lane 3: HL60 (Human) Cell Lysate at 30 ug
Primary:
Anti-Latency-associated peptide (bs-4908R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/35 kD
Observed band size: 50/33 kD

Sample:
MG63(Human) Cell Lysate at 30 ug
Primary: Anti-Latency-associated peptide (bs-4908R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30/44 kD
Observed band size: 30 kD

Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-Latency-associated peptide (bs-4908R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30/44 kD
Observed band size: 30 kD

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGFB1) Polyclonal Antibody, Unconjugated (bs-4908R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.