Anti-GABRA2 antibody
| 英文名称 | GABRA2 |
| 中文名称 | G氨基丁酸A型受体α2/GABAA Rα2抗体 |
| 别 名 | GABA A Receptor a2; GABA A Receptor alpha 2; GABA A receptor subunit alpha 2; GABA; GABA(A) receptor subunit alpha 2; GABA(A) receptor subunit alpha-2; GABR A2; GABRA 2; GABRA2; GABRA2 protein; Gamma aminobutyric acid (GABA) A receptor alpha 2; Gamma aminobutyric acid A receptor alpha 2; Gamma aminobutyric acid receptor alpha 2 subunit; Gamma aminobutyric acid receptor subunit alpha 2; Gamma-aminobutyric acid receptor subunit alpha-2; GBRA2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:GABRA2
IR:Immunogen Range:185-280/451
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2555
Swiss Prot:P47869
Source:KLH conjugated synthetic peptide derived from human GABRA2/GABA A Receptor alpha 2:185-280/451
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebral cortex(Rat) Lysate at 40 ug
Primary: Anti-GABRA2 (bs-12061R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GABRA2 (bs-12061R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample: Cerebrum (mouse) Lysate at 40 ug
Primary: Anti- GABRA2(bs-12061R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated(bs-12061R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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