Anti-GLUT2 antibody
英文名称 | GLUT2 |
中文名称 | 葡萄糖转运蛋白2抗体 |
别 名 | liver; Glucose Transporter 2; Glucose Transporter GLUT2; Glucose transporter type 2; Glucose transporter type 2 liver; GLUT-2; GLUT2; GLUT 2; GTR2_HUMAN; SLC2A2; Solute carrier family 2 (facilitated glucose transporter) member 2; Solute carrier family 2 facilitated glucose transporter member 2; Solute carrier family 2, facilitated glucose transporter member 2. |
DATASHEET
Host:Rabbit
Target Protein:GLUT2
IR:Immunogen Range:1-100/524
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6514
Swiss Prot:P11168
Source:KLH conjugated synthetic peptide derived from human GLUT2:1-100/524
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Glucose transporter 2 isoform is an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. It mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (bs-10379R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (bs-10379R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (bs-10379R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/45 kD
Observed band size: 47 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated(bs-10379R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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