Anti-LULL1/TOR1AIP2 antibody (bs-17326R)

Anti-LULL1/TOR1AIP2 antibody

DATASHEET

Host:Rabbit

Target Protein:LULL1/TOR1AIP2

IR:Immunogen Range:221-320/470

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:163590

Swiss Prot:Q8NFQ8

Source:KLH conjugated synthetic peptide derived from human LULL1:221-320/470 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:LULL1 is a 470 amino acid endoplasmic reticulum single-pass membrane protein belonging to the TOR1AIP family. LULL1 interacts with torsinA, an essential AAA+ ATPase found in the endoplasmic reticulum (ER) and nuclear envelope (NE) of higher eukaryotes. LULL1 regulates the distribution and activity of torsinA within the ER and NE lumen and reveals functional defects in mutant torsinA, which is responsible for DYT1 dystonia, a neurodevelopmental disease caused by an in-frame deletion (Deltagag) in the gene encoding torsinA. The gene encoding LULL1 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-LULL1/TOR1AIP2(bs-17326R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 56kD

Sample:
Placenta (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-LULL1/TOR1AIP2(bs-17326R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 56kD

Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: HUVEC (Human) Cell Lysate at 30 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Lane 6: HepG2 (Human) Cell Lysate at 30 ug
Lane 7: Liver (Mouse) Lysate at 40 ug
Lane 8: Liver (Rat) Lysate at 40 ug
Lane 9: Kidney (Mouse) Lysate at 40 ug
Lane 10: Cerebrum (Mouse) Lysate at 40 ug
Lane 11: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-LULL1/TOR1AIP2 (bs-17326R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51kD

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (bs-17326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (bs-17326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.