Anti-p21-ARC antibody (bs-17589R)

Anti-p21-ARC antibody

DATASHEET

Host:Rabbit

Target Protein:p21-ARC

IR:Immunogen Range:2-100/178

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10094

Swiss Prot:O15145

Source:KLH conjugated synthetic peptide derived from human p21-ARC:2-100/178 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Rabbit
Xenopus laevis.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: intestinal(mouse) lysate at 30ug;
Primary: rabbit Anti-p21-ARC (bs-17589R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:20 kD
Observed band size: 20 kD

Protein:
intestinal(mouse) lysate at 30ug;
Raji(human) lysate at 30ug;
Primary: rabbit Anti-p21-ARC (bs-17589R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:20 kD
Observed band size: 20 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- p21-ARC (bs-17589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-p21-ARC (bs-17589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD

Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p21-ARC) Polyclonal Antibody, Unconjugated (bs-17589R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.