Anti-phospho-MAK (Tyr159) antibody (bs-18634R)

Anti-phospho-MAK (Tyr159) antibody (bs-18634R)

DATASHEET

Host:Rabbit

Target Protein:phospho-MAK (Tyr159)

IR:Immunogen Range:TD(p-Y)VS

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4117

Swiss Prot:P20794

Source:KLH conjugated synthesised phosphopeptide derived from human MAK around the phosphorylation site of Tyr159:TD(p-Y)VS 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: k562(human) lysate at 40ug;
Primary: rabbit Anti-phospho-MAK (Tyr159) (bs-18634R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 71 kD
Observed band size: 71 kD

Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- phospho-MAK (Tyr159) (bs-18634R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 73 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- phospho-MAK (Tyr159) (bs-18634R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71/66 kD

Tissue/cell: human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated(bs-18634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated(bs-18634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining