Anti-DHRS2 antibody
| 英文名称 | DHRS2 |
| 中文名称 | 脱氢酶/还原酶家族成员2抗体 |
| 别 名 | Dehydrogenase/reductase (SDR family) member 2; Dehydrogenase/reductase member 2; Dehydrogenase/reductase SDR family member 2; Dicarbonyl reductase HEP27; DHRSⅡ; Hep27; HEP27 protein; Protein D; SDR family member; SDR25C1; Short chain alcohol dehydrogenase family member; Short chain dehydrogenase/reductase family 25C member 1; DHRS2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:DHRS2
IR:Immunogen Range:61-160/258
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10202
Swiss Prot:Q13268
Source:KLH conjugated synthetic peptide derived from human DHRS2:61-160/258
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Protein: Hela(human) lysates at 40ug;
Primary: rabbit Anti-DARC (bs-10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:27 kD
Observed band size: 30 kD
Protein: lung(mouse) lysates at 40ug; Primary: Anti-DHRS2 (bs-10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :27 kD
Observed band size :27 kD
Protein: liver(mouse) lysates at 30 ug;
Primary: Anti-DHRS2 (bs-10771R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 27 kD
Observed band size : 27/75 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(bs-10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(bs-10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度