Anti-VNN1 antibody
| 英文名称 | VNN1 |
| 中文名称 | 血管非炎症分子1抗体 |
| 别 名 | HDLCQ8; High density lipoprotein cholesterol level quantitative trait locus 8, included; Pantetheinase; Pantetheine hydrolase; Tiff66; V-1 antibody Vanin 1; Vanin-1; Vannin 1; Vascular non inflammatory molecule 1; Vascular non-inflammatory molecule 1; VNN 1; VNN1; VNN1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:VNN1
IR:Immunogen Range:151-250/513
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:8876
Swiss Prot:O95497
Source:KLH conjugated synthetic peptide derived from human VNN1:151-250/513
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq, Feb 2009]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2μg/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: placenta(Mouse) Lysate at 40 ug
Primary: Anti-VNN1(bs-10314R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VNN1 Polyclonal Antibody, Unconjugated(bs-10314R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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