Anti-PPP3CC antibody
| 英文名称 | PPP3CC |
| 中文名称 | 蛋白磷酸酶3调节亚基C抗体 |
| 别 名 | calcineurin A gamma; CALCINEURIN A3; Calcineurin; Calcineurin testis specific catalytic subunit; Calmodulin dependent calcineurin A subunit gamma isoform; Calmodulin-dependent calcineurin A subunit gamma isoform; CALNA3; CAM PRP catalytic subunit; CAM-PRP catalytic subunit; CNA3; pp2b gamma; ; PP2BC_HUMAN; PP2Bgamma; ; PPP3CC; protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform (calcineurin A gamma); protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform; Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform; testis-specific catalytic subunit. |
DATASHEET
Host:Rabbit
Target Protein:PPP3CC
IR:Immunogen Range:51-150/512
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5533
Swiss Prot:P48454
Source:KLH conjugated synthetic peptide derived from human PPP3CC:51-150/512
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Calcineurin is a calcium-dependent, calmodulin-stimulated protein phosphatase involved in the downstream regulation of dopaminergic signal transduction. Calcineurin is composed of a regulatory subunit and a catalytic subunit. The protein encoded by this gene represents one of the regulatory subunits that has been found for calcineurin. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Testis(Mouse)Lysate at 40 ug
Primary: Anti-PPP3CC(bs-19973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Sample: A549 (Human)Cell Lysate at 40 ug
Primary: Anti-PPP3CC(bs-19973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Sample:
Hela(Human) Cell Lysate at 40 ug
Primary: Anti-PPP3CC (bs-19973R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample:
DU145(Human) Cell Lysate at 40 ug
Primary: Anti-PPP3CC (bs-19973R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated (bs-19973R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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