Anti-Rad51 antibody (bs-20297R)

Anti-Rad51 antibody

DATASHEET

Host:Rabbit

Target Protein:Rad51

IR:Immunogen Range:81-180/339

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5888

Swiss Prot:Q06609

Source:KLH conjugated synthetic peptide derived from human Rad51:81-180/339 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:bone(mouse) Lysate at 40 ug
Primary: Anti-Rad51(bs-20297R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 37kD

Sample:Hela(human)cell Lysate at 40 ug
Primary: Anti-Rad51(bs-20297R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 37kD

Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (bs-20297R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (bs-20297R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (bs-20297R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: H9C2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (bs-20297R-AF555) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Tissue/cell: FHC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (bs-20297R-AF555) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.