Anti-ATPase Na+/ K+ beta 2(Loading Control) antibody (bs-23414R)

Anti-ATPase Na+/ K+ beta 2(Loading Control) antibody

DATASHEET

Host:Rabbit

Target Protein:ATPase Na+/ K+ beta 2(Loading Control)

IR:Immunogen Range:21-120/290

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:482

Swiss Prot:P14415

Source:KLH conjugated synthetic peptide derived from human ATPase Na+/ K+ beta 2:21-120/290 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-ATPase Na+/ K+ beta 2 (bs-23414R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD

Sample:
Bone (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti- ATPase Na+/ K+ beta 2 (bs-23414R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 31 kD

Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-ATPase Na+/ K+ beta 2 (bs-23414R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 73 kD

Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-ATPase Na+/ K+ beta 2 (bs-23414R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD

Sample:
A549(Human) Cell Lysate at 40 ug
Primary: Anti-ATPase Na+/ K+ beta 2 (bs-23414R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD