Anti-CK14+17+42+10 antibody
| 英文名称 | CK14+17+42+10 |
| 中文名称 | 细胞角蛋白14抗体 |
| 别 名 | Cytokeratin 14; CK 14; ck14; Cytokeratin14; Cytokeratin-14; Dowling Meara; ebs3; Epidermolysis bullosa simplex; k14; Keratin 14; Keratin type I cytoskeletal 14; Keratin14; Koebner; Krt 14; krt14; EBS3; EBS4; NFJ; Keratin, type I cytoskeletal 14; Cytokeratin 17; CK 17; ck17; Cytokeratin17; Cytokeratin-17; Keratin, type I cytoskeletal 17; Cytokeratin 10; CK 10; ck10; Cytokeratin10; Cytokeratin-10; Keratin, type I cytoskeletal 10; Cytokeratin 42; CK 42; ck42; Cytokeratin42; Cytokeratin-42; Keratin, type I cytoskeletal 42; K1C14_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:CK14+17+42+10
IR:Immunogen Range:411-472/472
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3861
Swiss Prot:P02533
Source:KLH conjugated synthetic peptide derived from human CK14+17+42+10:411-472/472
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-CK14+17+42+10 (bs-22150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-CK14+17+42+10 (bs-10196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Esophageal (Mouse) Lysate at 40 ug
Primary: Anti-CK14+17+42+10 (bs-22150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Uterus (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
Ear (Mouse) Lysate at 40 ug
Primary: Anti- CK14+17+42+10 (bs-22150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK14+17+42+10) Polyclonal Antibody, Unconjugated (bs-22150R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining
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