Anti-PD-L1 antibody (bs-1103R)

Anti-PD-L1 antibody

DATASHEET

Host:Rabbit

Target Protein:PD-L1

IR:Immunogen Range:201-290/290

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:60533

Swiss Prot:Q9EP73

Source:KLH conjugated synthetic peptide derived from mouse B7-H1:201-290/290 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Programmed cell death 1 ligand 1(Programmed death ligand 1；PD-L1; PDCD1 ligand 1; B7 homolog 1; B7-H1; CD274) involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibit T-cell proliferation and cytokine production.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti- CD274 (bs-1103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 32 kD
Observed band size: 48 kD

Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- CD274 (bs-1103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 32 kD
Observed band size: 48 kD

Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CD274 Polyclonal Antibody, Unconjugated(bs-1103R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining