Anti-BTG2 antibody (bs-0031R)

Anti-BTG2 antibody

DATASHEET

Host:Rabbit

Target Protein:BTG2

IR:Immunogen Range:31-110/158

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7832

Swiss Prot:P78543

Source:KLH conjugated synthetic peptide derived from human BTG2:31-110/158 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:BTG2 is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. BTG2 is involved in the regulation of the G1/S transition of the cell cycle. It modulates transcription regulation mediated by ESR1(referenced from Entrez Gene). BTG2 expression is induced in vivo during neurogenesis, and the gene is transiently expressed in vitro in rat pheochromocytoma PC12 cells after induction of neuronal differentiation by addition of nerve growth factor (NGF); suggesting that BTG2 is functionally significant during the neuronal differentiation process (PMID: 12360398).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Rabbit
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HepG2 (human)cell Lysate at 40 ug
Hcclm3 (human)cell Lysate at 40 ug
Primary: Anti- BTG2 (bs-0031R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17kD
Observed band size: 17 kD

Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-BTG2 (bs-0031R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 16 kD

Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BTG2 Polyclonal Antibody, Unconjugated(bs-0031R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining