Anti-Estrogen receptor beta antibody (bs-0116R)

Anti-Estrogen receptor beta antibody

DATASHEET

Host:Rabbit

Target Protein:Estrogen receptor beta

IR:Immunogen Range:201-300/530

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2100

Swiss Prot:Q92731

Source:KLH conjugated synthetic peptide derived from human ER-beta:201-300/530 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the family of estrogenreceptors and superfamily of nuclear receptor transcriptionfactors. The gene product contains an N-terminal DNA binding domainand C-terminal ligand binding domain and is localized to thenucleus, cytoplasm, and mitochondria. Upon binding to17beta-estradiol or related ligands, the encoded protein formshomo- or hetero-dimers that interact with specific DNA sequences toactivate transcription. Some isoforms dominantly inhibit theactivity of other estrogen receptor family members. Severalalternatively spliced transcript variants of this gene have beendescribed, but the full-length nature of some of these variants hasnot been fully characterized. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(3μg/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Testis (Rat) Lysate at 40 ug
Lane 3: Ovary (Mouse) Lysate at 40 ug
Lane 4: Uterus (Mouse) Lysate at 40 ug
Lane 5: Uterus (Rat) Lysate at 40 ug
Primary: Anti-Estrogen receptor beta (bs-0116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55-59 kD
Observed band size: 58-62 kD

Sample:
Kidney(Mouse) lysate at 30ug;
Brain(Rat) lysate at 30ug;
Primary: Anti-ER-beta (bs-0116R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
Predicted band size : 58kD
Observed band size : 58kD

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Estrogen receptor beta) Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.