Anti-GFP antibody (bs-0639R)

Anti-GFP antibody

DATASHEET

Host:Rabbit

Target Protein:GFP

IR:Immunogen Range:151-238/238

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:107331116

Swiss Prot:P42212

Source:KLH conjugated synthetic peptide derived from belt jellyfish GFP:151-238/238 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:20000-50000)
IF(1:100-500)

Cross Reactive Species:protein.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
GFP-Tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Primary:
Anti-GFP (bs-0639R) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 30 kD

Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Primary: Anti-GFP (bs-0639R) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD

Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 2 ug
Primary: Anti-GFP (bs-0639R) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD

Sample:
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti-GFP (bs-0639R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD