Anti-Integrin Alpha 3 + Beta 1 antibody (bs-1057R)

Anti-Integrin Alpha 3 + Beta 1 antibody

DATASHEET

Host:Rabbit

Target Protein:Integrin Alpha 3 + Beta 1

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3675

Swiss Prot:P05556

Source:KLH conjugated synthetic peptide derived from human Integrin Alpha 3(976-1025aa) + Beta 1(35-47aa): 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Lung (Mouse) Lysate at 30 ug
Primary: Anti- Integrin Alpha 3 + Beta 1 (bs-1057R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 130 kD

Sample: Cerebrum (Mouse) Lysate at 30 ug
Primary: Anti- Integrin Alpha 3 + Beta 1 (bs-1057R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 117 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin Alpha 3 + Beta 1) Polyclonal Antibody, Unconjugated (bs-1057R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.