Anti-Myelin Protein Zero antibody (bs-0337R)

Anti-Myelin Protein Zero antibody

DATASHEET

Host:Rabbit

Target Protein:Myelin Protein Zero

IR:Immunogen Range:175-248/248

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4359

Swiss Prot:P25189

Source:KLH conjugated synthetic peptide derived from human P0 protein:175-248/248 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Myelin protein-zero is the major structural protein of peripheral myelin, accounting for more than 50% of the protein present in the sheath of peripheral nerves. Expression of the gene encoding Myelin protein-zero (MPZ) is restricted to Schwann cells; MPZ is not found in the CNS. An integral membrane glycoprotein of ~28kDa, Myelin protein-zero is thought to link adjacent lamellae and thereby stabilize the myelin assembly. The other 3 major components of myelin are myelin basic protein, myelin proteolipid protein and myelin-associated glycoprotein.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Spinal cord (Rat) Lysate at 40 ug
Primary: Anti- Myelin Protein Zero (bs-0337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 29 kD

Sample:
Lane 1: Spinal cord (Mouse) Lysate at 40 ug
Lane 2: Spinal cord (Rat) Lysate at 40 ug
Lane 3: Adipose (Mouse) Lysate at 40 ug
Lane 4: Adipose (Rat) Lysate at 40 ug
Primary: Anti-Myelin Protein Zero (bs-0337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD

Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-Myelin Protein Zero (Bs- 0337R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD