Anti-P-cadherin antibody
| 英文名称 | P-cadherin |
| 中文名称 | P-钙粘附分子抗体 |
| 别 名 | placental; Cadherin-3; P cadherin; Cadp; Placental cadherin; P-cadherin; AI385538; Pca; Cadherin 3 precursor; Cadherin 3 type 1; Calcium dependent adhesion protein placental; Cadherin; CDH3; CDHP; 7B4 antigen; Cadherin-1; Cadherin-2; Cadherin-4; Cadherin-5; CAM 120/80; CD144; CD324; CD325; Cdh4; CDH5; CDHE; CDHN; CADH3_HUMAN; E-cadherin; Epithelial cadherin; N-cadherin; NCAD; Neural cadherin; P-cadherin; UVO; Uvomorulin; Vascular endothelial cadherin; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; PCAD; Placental cadherin. |
DATASHEET
Host:Rabbit
Target Protein:P-cadherin
IR:Immunogen Range:625-725/829
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1001
Swiss Prot:P22223
Source:KLH conjugated synthetic peptide derived from human P-cadherin:625-725/829
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2μg/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-P-cadherin (bs-1159R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 90 kD
Tissue/cell: U251 cells;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated(bs-1159R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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