Anti-Nogo B antibody
| 英文名称 | Nogo B |
| 中文名称 | 轴索过度生长抑制因子B抗体 |
| 别 名 | reticulon-4 isoform B; RTN4; RTN4-B1; Nbla10545; RTN-X; reticulon-4A; Nogo-C; NSP; RTN-x; ASY; foocen; NSP-CL; NOGOC; RTN4-A; Reticulon-4; Nbla00271; KIAA0886; NOGOA; Reticulon-5; Foocen; NI220/250; RTN4-C; RTN4-B2; SP1507; NOGO-A; NOGOB; Nogo-B; Nogo-B/A. |
DATASHEET
Host:Rabbit
Target Protein:Nogo B
IR:Immunogen Range:251-357/357
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:57142
Swiss Prot:Q99P72
Source:KLH conjugated synthetic peptide derived from human reticulon-4 isoform B:251-357/357
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Cerebellum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Rat) Lysate at 40 ug
Primary:
Anti-Nogo B (bs-1315R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 45 kD
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Nogo A+B (bs-1315R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nogo-B Polyclonal Antibody, Unconjugated(bs-1315R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度