Anti-Cyclin G antibody (bs-1389R)

Anti-Cyclin G antibody

DATASHEET

Host:Rabbit

Target Protein:Cyclin G

IR:Immunogen Range:181-295/295

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:900

Swiss Prot:P51959

Source:KLH conjugated synthetic peptide derived from human Cyclin G:181-295/295 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Cyclin G contains a typical N terminal cyclin box and a carboJNC terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD

Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin G Polyclonal Antibody, Unconjugated(bs-1389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining