Anti-HDAC1 (Nuclear Loading Control) antibody
| 英文名称 | HDAC1 (Nuclear Loading Control) |
| 中文名称 | 组蛋白去乙酰化酶1（核内参）抗体 |
| 别 名 | DKFZp686H12203; GON 10; HD 1; HD1; HDAC 1; hdac1: histone deacetylase 1; Histone deacetylase 1 (HD1); Histone deacetylase 1; RPD3 (reduced potassium dependency yeast homolog) like 1; RPD3 (reduced potassium dependency); RPD3; RPD3L1; HDAC1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:HDAC1 (Nuclear Loading Control)
IR:Immunogen Range:381-482/482
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3065
Swiss Prot:Q13547
Source:KLH conjugated synthetic peptide derived from human HDAC1:381-482/482
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1:50-300)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-HDAC1 (bs-1414R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample:
Panc-1(Human) Cell Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Hela(Human) Lysate at 40 ug
Primary: Anti-HDAC1 (bs-1414R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HDAC1 Polyclonal Antibody, Unconjugated(bs-1414R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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