Anti-CD68 antibody (bs-1432R)

Anti-CD68 antibody 

DATASHEET

Host:Rabbit

Target Protein:CD68

IR:Immunogen Range:1-130/354

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:968

Swiss Prot:P34810

Source:KLH conjugated synthetic peptide derived from human CD68:1-130/354 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 2: Lymph node (Rat) Cell Lysate at 30 ug
Primary: Anti-CD68 (bs-1432R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/185-200 kD
Observed band size: 110 kD

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: Jurkat (Human) Cell Lysate at 30 ug
Lane 4: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-CD68 (bs-1432R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/185-200 kD
Observed band size: 110 kD

Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(bs-1432R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

This image was generously provided by Amanda Brown, Ph.D. from Johns Hopkins University School of Medicine. Paraffin embedded human brain tissue labeled with Rabbit Anti-CD68 Polyclonal Antibody, Unconjugated (bs-1432R) at 1:50 followed by conjugation to the secondary antibody and Permanent Fast Red Quanto staining