Anti-ABP antibody
| 英文名称 | ABP |
| 中文名称 | 雄激素结合蛋白抗体 |
| 别 名 | Androgen binding protein; MGC126834; MGC138391; SBP; Sex steroid binding protein; SHBG; TEBG; Testis specific androgen binding protein; Testosterone binding beta globulin; Testosterone Estradiol Binding Globulin; Testosterone estrogen binding globulin; UniProtKB/Swiss-Prot: P04278.2; SHBG_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:ABP
IR:Immunogen Range:51-150/402
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6462
Swiss Prot:P04278
Source:KLH conjugated synthetic peptide derived from human ABP:51-150/402
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Sex hormone binding globulin (SHBG) is a glycoprotein synthesized by the liver. Circulating androgen and estrogen concentrations influence SHBG synthesis. Elevated testosterone, for example, causes SHBG synthesis to decrease, whereas high estrogen stimulates SHBG production. The regulation of SHBG synthesis, combined with SHBG's higher affinity for testosterone, impacts bioavailable testosterone levels. SHBG binds up to 98 percent of the steroid hormones in the blood including 5a-dihydrotestosterone (DHT), testosterone and androstenediol with particularly high affinity, and estradiol and estrone with slightly lower affinity.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane1: Testis (Mouse) Lysate at 30 ug
Lane2: Liver (Mouse) Lysate at 30 ug
Primary: Anti-ABP/SHBG(bs-2410R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 40kD
Observed band size : 44kD
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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