Anti-Amyloid Precursor Protein antibody
| 英文名称 | Amyloid Precursor Protein |
| 中文名称 | APP/ABPP淀粉样肽前体蛋白抗体 |
| 别 名 | Soluble APP-alpha; Soluble APP-beta; A4 amyloid protein; A4; AAA; ABETA; ABPP; AD 1; AD1; Alzheimer disease 1; Alzheimer disease; Alzheimer's disease amyloid protein; Amyloid beta (A4) precursor protein; Amyloid beta A4 protein; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform a; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta protein; Amyloid beta-peptide; Amyloid of aging and alzheimer disease; APP; APP I; APPI; Beta amyloid peptide; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Human mRNA for amyloid A4 precursor of Alzheimer's disease; PN 2; PN II; PN2; PreA4; Protease nexin II; A4_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Amyloid Precursor Protein
IR:Immunogen Range:381-480/770
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:351
Swiss Prot:P05067
Source:KLH conjugated synthetic peptide derived from of human APP:381-480/770
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
A431 Cell (Human) Lysate at 40 ug
Raji Cell (Human) Lysate at 40 ug
Primary: Anti- Amyloid Precursor Protein (bs-0112R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72-83 kD
Observed band size: 75 kD
Sample:
Eye (Rat) Lysate at 40 ug
Primary: Anti- Amyloid Precursor Protein (bs-0112R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72-83 kD
Observed band size: 75 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amyloid Precursor Protein Polyclonal Antibody, Unconjugated(bs-0112R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Amyloid Precursor Protein) polyclonal Antibody, Unconjugated (bs-0112R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
好评度