Anti-NeuroD1 antibody
| 英文名称 | NeuroD1 |
| 中文名称 | 神经细胞分化因子1抗体 |
| 别 名 | atonal; Neurod1 protein; basic helix loop helix transcription factor; bHLHa3; class A basic helix loop helix protein 3; Class A basic helix-loop-helix protein 3; MODY 6; MODY6; NDF1_HUMAN; NeuroD1; neurogenic helix loop helix protein NEUROD; Beta cell E box transactivator 2; BETA2; BHF 1; BHF1; NEUROD; Neurogenic differentiation 1; Neurogenic differentiation factor 1; NIDDM; BHLHA3; NEUROD1. |
DATASHEET
Host:Rabbit
Target Protein:NeuroD1
IR:Immunogen Range:21-120/356
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4760
Swiss Prot:Q13562
Source:KLH conjugated synthetic peptide derived from human NeuroD1:21-120/356
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(0.2ug/test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Stomach (Mouse) Lysate at 40 ug
Lane 2: Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-NeuroD1 (bs-1517R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Lane 1: Cerebellum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- NeuroD1 (bs-1517R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NeuroD1 Polyclonal Antibody, Unconjugated(bs-1517R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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