Anti-Elastin antibody (bs-1756R)

Anti-Elastin antibody

DATASHEET

Host:Rabbit

Target Protein:Elastin

IR:Immunogen Range:681-786/786

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2006

Swiss Prot:P15502

Source:KLH conjugated synthetic peptide derived from human Elastin:681-786/786 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Uterus (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Liver (Mouse) Lysate at 40 ug
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 5: Lung (Rat) Lysate at 40 ug
Lane 6: Liver (Rat) Lysate at 40 ug
Lane 7: Testis (Rat) Lysate at 40 ug
Lane 8: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 9: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Elastin (bs-1756R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 68 kD

Sample: Lung (Mouse) Lysate at 30 ug
Primary: Anti- alpha Elastin/Tropoelastin (bs-1756R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 70 kD
Observed band size: 68 kD

Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (bs-1756R) at 1:1000 4℃ temperature overnight 4℃followed by conjugation to secondary antibody.