Anti-SLC10A1 antibody
| 英文名称 | SLC10A1 |
| 中文名称 | 钠离子/牛磺胆酸共转运蛋白抗体 |
| 别 名 | Na+/taurocholate Cotransporting Polypeptide; Na(+)/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na / bile acid cotransporter; Na / taurocholate transport protein; Na(+)/taurocholate transport protein; NTCP; NTCP1; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1; Slc10a1 protein; Slc10a1; SBACT; SLC10A1; NTCP_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:SLC10A1
IR:Immunogen Range:21-120/349
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6554
Swiss Prot:Q14973
Source:KLH conjugated synthetic peptide derived from human NTCP:21-120/349
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization(SLC10A2; MIM 601295), and the other being found in the basolateral membranes of hepatocytes (SLC10A1).[supplied by OMIM]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg /test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: K562 (human)cell Lysate at 40 ug
Primary: Anti- SLC10A1 (bs-1958R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 48 kD
Tissue/cell: Siha cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SLC10A1) Polyclonal Antibody, Unconjugated (bs-1958R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Tissue/cell: HT1080 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SLC10A1) Polyclonal Antibody, Unconjugated (bs-1958R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
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