Anti-DEP1 antibody
| 英文名称 | DEP1 |
| 中文名称 | 蛋白酪氨酸磷酸酶CD148抗体 |
| 别 名 | CD 148; CD148; CD148 antigen; Density enhanced phosphatase 1; Density-enhanced phosphatase 1; DEP 1; DEP-1; HPTP eta; HPTPeta; Human density enhanced phosphatase 1; Protein tyrosine phosphatase eta; Protein tyrosine phosphatase receptor type J; Protein tyrosine phosphatase receptor type J polypeptide; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPJ; Ptprj; PTPRJ_HUMAN; R PTP Eta; R-PTP-eta; R-PTP-J; Receptor type tyrosine protein phosphatase eta; Receptor-type tyrosine-protein phosphatase eta; SCC 1; SCC1. |
DATASHEET
Host:Rabbit
Target Protein:DEP1
IR:Immunogen Range:865-970/1337
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5795
Swiss Prot:Q12913
Source:KLH conjugated synthetic peptide derived from human PTPRJ:865-970/1337
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lymph node (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-DEP1 (bs-2567R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60'100 kD
Observed band size: 60 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-DEP1 (bs-2567R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60'100 kD
Observed band size: 60 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PTP/PTPase/CD148 Polyclonal Antibody, Unconjugated(bs-2567R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PTP/PTPase/CD148 Polyclonal Antibody, Unconjugated(bs-2567R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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