Anti-phospho-GCN2 (Thr667) antibody (bs-3156R)

Anti-phospho-GCN2 (Thr667) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-GCN2 (Thr667)

IR:Immunogen Range:PG(p-T)PP

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:440275

Swiss Prot:Q9P2K8

Source:KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667:PG(p-T)PP 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170/181 kD

Sample:
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.