Anti-phospho-PYK2 (Tyr881) antibody
| 英文名称 | phospho-PYK2 (Tyr881) |
| 中文名称 | 磷酸化富含脯氨酸的酪氨酸激酶2抗体 |
| 别 名 | PYK2 (phospho Tyr881); PYK2 (phospho Y881); p-PYK2 (Tyr881); p-PYK2 (Y881); CADTK; CAK beta; CAKB antibody; CAKbeta; Calcium dependent tyrosine kinase; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; E430023O05Rik; EC 2.7.10.2; FADK 2; FADK2; FAK 2; FAK1; FAK2; Focal Adhesion Kinase 2; MGC124628; PKB; pp125FAK; Proline Rich Tyrosine Kinase 2; Proline-rich tyrosine kinase 2; Protein kinase B; Protein Tyrosine Kinase 2 Beta; PTK 2B; PTK antibody PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK 2; PYK2; RAFTK; Related adhesion focal tyrosine kinase; FAK2_HUMAN; PTK2B protein tyrosine kinase 2 beta. |
DATASHEET
Host:Rabbit
Target Protein:phospho-PYK2 (Tyr881)
IR:Immunogen Range:LV(p-Y)LN
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2185
Swiss Prot:Q14289
Source:KLH conjugated synthesised phosphopeptide derived from human Pyk2 around the phosphorylation site of Tyr881:LV(p-Y)LN
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-phospho-PYK2 (Tyr881) (bs-3401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-phospho-PYK2 (Tyr881) (bs-3401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD
Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PYK2(Tyr881) Polyclonal Antibody, Unconjugated(bs-3401R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PYK2 (Tyr881)) Polyclonal Antibody, Unconjugated (bs-3401R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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