Anti-CTDSPL antibody (bs-3509R)

Anti-CTDSPL antibody

DATASHEET

Host:Rabbit

Target Protein:CTDSPL

IR:Immunogen Range:145-250/276

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10217

Swiss Prot:O15194

Source:KLH conjugated synthetic peptide derived from human SCP3:145-250/276 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:During meiosis, the homologous chromosomes pair and recombine, a process essential for generating genetically distinct haploid cells. The unique behavior displayed by meiotic chromosomes has been linked to the activities of a meiosis-specific supramolecular proteinaceous structure, the synaptonemal complex (SC). Three meiosis-specific components of the SC have been characterized in mammals, SCP1, SCP2 and SCP3. SCP3 has a C-terminal coiled-coil domain that has been shown to promote homotypic interactions in vitro.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-CTDSPL  (bs-3509R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 35 kD

Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTDSPL) Polyclonal Antibody, Unconjugated (bs-3509R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTDSPL) Polyclonal Antibody, Unconjugated (bs-3509R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTDSPL) Polyclonal Antibody, Unconjugated (bs-3509R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.