Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) antibody (bs-5181R)

Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-AXL (Tyr698+Tyr702+Tyr703)

IR:Immunogen Range:KI(p-Y)NGD(p-Y)(p-Y)RQ

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:558

Swiss Prot:P30530

Source:KLH conjugated Synthesised phosphopeptide derived from human AXL around the phosphorylation site of Tyr698+Tyr702+Tyr703:KI(p-Y)NGD(p-Y)(p-Y)RQ 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma.The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (bs-5181R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140’120 kD
Observed band size: 120 kD

Sample: SW480 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (bs-5181R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 120 kD

Sample:MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (bs-5181R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 120 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-AXL(Tyr698+Tyr702+Tyr703) Polyclonal Antibody, Unconjugated (bs-5181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining