Anti-MHC Class II/HLA DMB antibody
| 英文名称 | MHC Class II/HLA DMB |
| 中文名称 | 组织相容性复合体2抗体 |
| 别 名 | HLA-DPB1; MHC-2; MHC2; MHC 2; DMA; DMB; DP beta 1; DPB 1; DPB1; DRB; HLA class II histocompatibility antigen DM beta chain; HLA DMB; HLA DPB1; HLADM; HLADP1B; Major histocompatibility complex class II; Major histocompatibility complex class II DP beta 1; MHC class II antigen DMB; MHC DPB1; RING6; RING7; DPB1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:MHC Class II/HLA DMB
IR:Immunogen Range:101-210/258
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3115
Swiss Prot:P04440
Source:KLH conjugated synthetic peptide derived from human DPB1:101-210/258
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(0.2ug/test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-HLA DMB (bs-4107R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-MHC Class II/HLA DMB (bs-4107R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MHC Class II/HLA-DPB1 Polyclonal Antibody, Unconjugated(bs-4107R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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