Anti-phospho-NFKB p65 (Thr505) antibody
| 英文名称 | phospho-NFKB p65 (Thr505) |
| 中文名称 | 磷酸化细胞核因子抗体 |
| 别 名 | NFκ B-p65 (Phospho-Thr505); NFκ B-p65 (Phospho-Thr505); RELA(phospho T505); NF kB P65; NF-kB p65; NFKBp65; NF-κBp65; p65 NF kappaB; p65 NFkB; NFKBp65; RELA; Transcription Factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)); V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A; p65NFKB; Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NFKB 3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells. |
DATASHEET
Host:Rabbit
Target Protein:phospho-NFKB p65 (Thr505)
IR:Immunogen Range:LV(p-T)G
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5970
Swiss Prot:Q04206
Source:KLH conjugated synthesised phosphopeptide derived from human around the phosphorylation site of Thr505:LV(p-T)G
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Pancreas(Mouse) Lysate at 40 ug
HL60(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-NFKB p65 (Thr505) (bs-5663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample:
Molt-4(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-phospho-NFKB p65 (Thr505) (bs-5663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 62 kD
Sample:
Hcclm3 Cell Lysate at 30 ug
Primary: Anti-phospho-NFKB p65(Thr505) (bs-5663R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61/58 kD
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