Anti-ADAM8 antibody
| 英文名称 | ADAM8 |
| 中文名称 | 去整合素样金属蛋白酶8抗体 |
| 别 名 | A Disintegrin And Metalloproteinase domain 8; A Disintegrin And Metalloproteinase domain 8; ADAM 8; ADAM 8 precursor; ADAM 8 precursor; ADAM metallopeptidase domain 8; ADAM8 protein; CD 156; CD156; CD156a; CD156a antigen; CD156a antigen; Cell surface antigen MS2; Cell surface antigen MS2; Human leukocyte differentiation antigen; ADAM8_HUMAN; Human leukocyte differentiation antigen; Macrophage cysteine rich glycoprotein; Macrophage cysteine rich glycoprotein; MGC134985; MS 2; MS2. |
DATASHEET
Host:Rabbit
Target Protein:ADAM8
IR:Immunogen Range:51-150/824
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:101
Swiss Prot:P78325
Source:KLH conjugated synthetic peptide derived from human ADAM8 52-91aa:51-150/824
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
Flow-Cyt(1μg/Test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control: Mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Black line : Positive blank control A431); Negative blank control (HepG2）
Green line : Primary Antibody (Rabbit Anti-ADAM8 antibody (bs-4195R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
A431（Positive）and HepG2（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody(bs-4195R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Black line : Positive blank control A431); Negative blank control (HepG2）
Green line : Primary Antibody (Rabbit Anti-ADAM8 antibody (bs-4195R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
A431（Positive）and HepG2（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody(bs-4195R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ADAM8 antibody (bs-4195R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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