Anti-WNT2 antibody (bs-6133R)

Anti-WNT2 antibody

DATASHEET

Host:Rabbit

Target Protein:WNT2

IR:Immunogen Range:221-320/360

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7472

Swiss Prot:P09544

Source:KLH conjugated synthetic peptide derived from human WNT2:221-320/360 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti- WNT2 (bs-6133R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 37 kD
Observed band size: 38 kD

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT2) Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.