Anti-phospho-MCK10 (Tyr513) antibody
| 英文名称 | phospho-MCK10 (Tyr513) |
| 中文名称 | 磷酸化神经上皮酪氨酸激酶/乳腺癌激酶10抗体 |
| 别 名 | MCK10 (phospho Y513); p-MCK10 (phospho Y513); CAK; CD167a; DDR1CD 167; CD167; Cell adhesion kinase; DDR 1; DDR; DDR1; Discoidin domain receptor; Discoidin receptor tyrosine kinase isoform a; EDDR 1; Epithelial discoidin domain receptor 1; Epithelial specific receptor kinase; Mammarian carcinoma kinase 10; Neuroepithelial tyrosine kinase; Neurotrophic tyrosine kinase receptor type 4; NTRK 4; PTK 3; PTK 3A protein tyrosine kinase 3A; Receptor tyrosine kinase NEP; RTK 6; TRK E; Tyrosine kinase receptor E; DDR1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-MCK10 (Tyr513)
IR:Immunogen Range:PA(p-Y)RL
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:780
Swiss Prot:Q08345
Source:KLH conjugated synthesised phosphopeptide derived from human MCK10 around the phosphorylation site of Tyr513:PA(p-Y)RL
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MCK10 (Tyr513) (bs-6269R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 120 kD
Sample:
K562(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MCK10 (Tyr513) (bs-6269R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 120 kD
Sample:
K562(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-MCK10 (bs-6269R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 120 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-MCK10 (bs-6269R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 120 kD
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