Anti-EDG3 antibody
| 英文名称 | EDG3 |
| 中文名称 | 内皮分化型G蛋白偶联受体3抗体 |
| 别 名 | EDG 3; EDG3; Endothelial differentiation G protein coupled receptor 3; Endothelial differentiation G-protein coupled receptor 3; Endothelial differentiation gene 3; Endothelial differentiation sphingolipid G protein coupled receptor 3; FLJ37523; FLJ93220; G protein coupled receptor endothelial differentiation gene 3; LPB 3; LPB3; S1P receptor 3; S1P receptor Edg 3; S1P receptor Edg-3; S1P receptor EDG3; S1P3; S1PR3; S1PR3_HUMAN; Sphingosine 1 phosphate receptor 3; Sphingosine 1 phosphate receptor Edg 3; Sphingosine 1 phosphate receptor Edg3; Sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3. |
DATASHEET
Host:Rabbit
Target Protein:EDG3
IR:Immunogen Range:145-250/378
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1903
Swiss Prot:Q99500
Source:KLH conjugated synthetic peptide derived from human EDG3:145-250/378
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(0.2u/test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane1: Heart (Mouse) Lysate at 40 ug
Primary: Anti-EDG3 (bs-7541R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 42 kD
Observed band size: 46 kD
Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Lane 4: Heart (Mouse) Lysate at 40 ug
Primary: Anti-EDG3 (bs-7541R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-EDG3 Polyclonal Antibody, Unconjugated(bs-7541R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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