Anti-Porimin antibody (bs-7674R)

Anti-Porimin antibody

DATASHEET

Host:Rabbit

Target Protein:Porimin

IR:Immunogen Range:101-180/208

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:114908

Swiss Prot:Q8N131

Source:KLH conjugated synthetic peptide derived from human Porimin:101-180/208 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti PORIMIN (pro oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Skin (Mouse) Lysate at 40 ug
Skin (Rat) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 64 kD

Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 74 kD

Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 74 kD