Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) antibody (bs-7913R)

Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-LATS1+LATS2 (Thr1079 +Thr1041)

IR:Immunogen Range:EF(P-T)FR

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:26524

Swiss Prot:O95835

Source:KLH conjugated synthesised phosphopeptide derived from human LATS1 around the phosphorylation site of Thr1079:EF(P-T)FR 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (bs-7913R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (bs-7913R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-LATS1+LATS2 (Thr1079 +Thr1041)) Polyclonal Antibody, Unconjugated (bs-7913R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.