Anti-phospho-GluR1 (Ser849) antibody
| 英文名称 | phospho-GluR1 (Ser849) |
| 中文名称 | 磷酸化谷氨酸受体1抗体 |
| 别 名 | GluR1 (phospho Ser849); GluR1 (phospho S849); p- GluR1(Ser849); p- GluR1(S849); GLUR 1; GLUR A; AMPA 1; AMPA selective glutamate receptor 1; GLUH 1; GLUH1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA ; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Gria 1; Gria1 ; Gria1; HBGR1; MGC133252. |
DATASHEET
Host:Rabbit
Target Protein:phospho-GluR1 (Ser849)
IR:Immunogen Range:QQ(p-S)IN
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2890
Swiss Prot:P42261
Source:KLH conjugated synthesised phosphopeptide derived from human GluR1 around the phosphorylation site of Ser849:QQ(p-S)IN
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroJNC-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
U251 (Human) Lysate at 30 ug
Primary: Anti-phospho-GluR1 (Ser849) (bs-8446R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 128 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-GluR1 (Ser849) (bs-8446R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 128 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Cerebellum (Rat) Lysate at 40 ug
U251 (Human) Lysate at 30 ug
Primary: Anti- phospho-GluR1 (Ser849) (bs-8446R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 130 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GluR1 (Ser849)) Polyclonal Antibody, Unconjugated (bs-8446R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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