Anti-KATNA1 antibody (bs-9308R)

Anti-KATNA1 antibody

DATASHEET

Host:Rabbit

Target Protein:KATNA1

IR:Immunogen Range:221-320/491

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:11104

Swiss Prot:O75449

Source:KLH conjugated synthetic peptide derived from human KATNA1/Katanin p60 A1:221-320/491 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Microtubules are polymers of alpha and beta subunits that form the mitotic spindle and assist in the organization of membranous organelles during interphase. Katanin p60 A1, also known as KATNA1, is a 491 amino acid protein that belongs to the AAA ATPase family and is involved in microtubule regulation. Localized to the cytoplasm and to the centrosome, Katanin p60 A1 functions to sever and disassemble microtubules in an ATP-dependent manner, thus promoting the rapid reorganization of cellular microtubule arrays and playing an important role in microtubule release from the centrosome after nucleation. Katanin p60 A1, which exists as two alternatively spliced isoforms, can homooligomerize into hexameric rings whose activity is stimulated by the presence of microtubules.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Fetal brain (Mouse) Lysate at 40 ug
Primary: Anti-KATNA1 (bs-9308R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:56 kD
Observed band size: 56 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-KATNA1 (bs-9308R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:56 kD
Observed band size: 56 kD

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (bs-9308R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (bs-9308R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.