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Anti-GTPBP9 antibody (bs-9554R)

Anti-GTPBP9 antibody (bs-9554R)

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Anti-GTPBP9 antibody

英文名称GTPBP9
中文名称鸟嘌呤核苷酸结合蛋白9抗体
别    名DNA damage-regulated overexpressed in cancer 45 protein; DOC45; GBP45; GTP-binding protein 9 (putative); GTP-binding protein 9; GTP-binding protein PTD004; homologous yeast-44.2 protein; Obg like ATPase 1; Obg-like ATPase 1; OLA1; OLA1_HUMAN; PTD004.  

DATASHEET

Host:Rabbit

Target Protein:GTPBP9

IR:Immunogen Range:151-250/396

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:29789

Swiss Prot:Q9NTK5

Source:KLH conjugated synthetic peptide derived from human GTPBP9:151-250/396 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:GTP-binding protein 9 is a 396 amino acid protein that belongs to the Obg-related GTPase family under the translation factors (TRAFAC) class. Originally thought to only have GTPase activity, Obg-related GTPase family members have been shown to also have ATPase activity. In Homo sapians, GTPBP9 exhibits a preference for binding ATP over GTP, with GTP binding occuring only at high nucleotide concentration. One cause for ATP affinity and GTP discrimination is thought to be a substitution of glutamine for a hydrophobic amino acid in Obg-related family members; this is the same substitution that inactivates Ras-like GTPases. GTPBP9 contains a C-terminal TGS domain that binds to ligands and an N-terminal G domain which binds nucleotides. GTPBP9 is expressed as three isoforms produced by alternative splicing.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Sheep
Fruit Fly
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Raji Cell (Human) Lysate at 30 ug
Primary: Anti-GTPBP9 (bs-9554R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD

Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-GTPBP9 (bs-9554R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD

Sample:
HepG2 Cell (Human) Lysate at 30 ug
TT Cell (Human) Lysate at 30 ug
Primary: Anti-GTPBP9 (bs-9554R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD

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